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psd95  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc psd95
    Modulation of synaptic plasticity protein expression by L-lactate and GPR81 agonist in primary hippocampal cells. (a) Exemplary immunoblotting analysis. (b-d) Bar graphs depict the quantified protein levels (normalized to CON) for BDNF, SYN, and <t>PSD95,</t> derived from three independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01 versus the CON group.
    Psd95, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+psd95/PSD95+XP+Rabbit+mAb/pmc13658191-116-36-38
    Average 96 stars, based on 654 article reviews
    psd95 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    1) Product Images from "Lactate promotes mitochondrial biogenesis and fusion via GPR81 to enhance synaptic plasticity-related protein expression in hippocampal cells"

    Article Title: Lactate promotes mitochondrial biogenesis and fusion via GPR81 to enhance synaptic plasticity-related protein expression in hippocampal cells

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2026.1929409

    Modulation of synaptic plasticity protein expression by L-lactate and GPR81 agonist in primary hippocampal cells. (a) Exemplary immunoblotting analysis. (b-d) Bar graphs depict the quantified protein levels (normalized to CON) for BDNF, SYN, and PSD95, derived from three independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01 versus the CON group.
    Figure Legend Snippet: Modulation of synaptic plasticity protein expression by L-lactate and GPR81 agonist in primary hippocampal cells. (a) Exemplary immunoblotting analysis. (b-d) Bar graphs depict the quantified protein levels (normalized to CON) for BDNF, SYN, and PSD95, derived from three independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01 versus the CON group.

    Techniques Used: Expressing, Western Blot, Analysis, Derivative Assay

    Effects of mitochondrial dysfunction on expression of genes and proteins related to synaptic plasticity in Neuro-2A cells. (a-e) Quantitative analysis (qRT-PCR) of Arc , Fos , Egr1 , bdnf , and Syp , derived from three independent replicates. (f) Exemplary Western blot image. (g-i) Bar graphs depict the quantified protein levels for BDNF, SYN, and PSD95, derived from six independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the LA group. # p < 0.05, ## p < 0.01, ### p < 0.001 versus the 3OBA group. n. s., no significant difference.
    Figure Legend Snippet: Effects of mitochondrial dysfunction on expression of genes and proteins related to synaptic plasticity in Neuro-2A cells. (a-e) Quantitative analysis (qRT-PCR) of Arc , Fos , Egr1 , bdnf , and Syp , derived from three independent replicates. (f) Exemplary Western blot image. (g-i) Bar graphs depict the quantified protein levels for BDNF, SYN, and PSD95, derived from six independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the LA group. # p < 0.05, ## p < 0.01, ### p < 0.001 versus the 3OBA group. n. s., no significant difference.

    Techniques Used: Expressing, Analysis, Quantitative RT-PCR, Derivative Assay, Western Blot

    Related Articles

    Incubation:

    Article Title: Retrosplenial cortex vulnerability links severe hypoglycemia to cognitive impairment through neuron–microglia crosstalk
    Article Snippet: Equal amounts of protein were separated on 8% or 10% sodium dodecyl sulfate‒polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Millipore, IPVH00010). .. The membranes were blocked with 5% skim milk for one hour at room temperature, followed by overnight incubation at 4°C with primary antibodies [anti-IL-1β (Proteintech, 16806-1-AP, 1:500), anti-TNF-α (Abcam, ab66579, 1:500), anti-IL-6 (Cell Signaling Technology, 12912S, 1:500), anti-Drp1 (Santa Cruz Biotechnology, SC-271583, 1:1,000), anti-phospho-Drp1 (Ser616) (Cell Signaling Technology, 3455S, 1:1,000), anti-PSD95 (Cell Signaling Technology, 3450S, 1:1,000), anti-synaptophysin (Sigma‒Aldrich, S5768, 1:500), anti-Mfn1 (ABclonal, A9880, 1:1,000), anti-Mfn2 (ABclonal, A19678, 1:1,000), anti-OPA1 (ABclonal, A9833, 1:1,000), anti-VDAC1 (Abcam, ab14734, 1:1,000), and anti-β-actin (Santa Cruz Biotechnology, SC-81178, 1:3,000)]. .. After three washes, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies [goat anti-mouse (Invitrogen, 31430, 1:2,000) and goat anti-rabbit (Invitrogen, 31460, 1:2,000)] for one hour at room temperature.

    Article Title: Targeting AARS1-dependent lactylation improves neuronal process plasticity and mitigates cognitive deficits in sepsis-associated encephalopathy.
    Article Snippet: Sepsis-associated encephalopathy (SAE) is a frequent and severe neurological complication of systemic infection, strongly associated with long-term cognitive impairment.1 Despite its clinical significance, no targeted therapies are currently available.. In this study, we established a murine model of sepsis using cecal ligation and puncture (CLP) and identified a metabolic pathway in which lactate accumulation drives neuronal process injury through aberrant activation of the ATRIP–ATR signaling axis.. During sepsis, AARS1—the primary lactyltransferase responsible for lactylation in neuronal processes—catalyzes the lactylation of ATRIP at lysine 127 (K127), triggering ATRIP–ATR complex formation and pathway activation independent of DNA damage.

    Membrane:

    Article Title: Reduced astrocytic Homer1a expression mediates mPFC excitatory synaptic dysfunction and neurodevelopmental deficits after maternal immune activation
    Article Snippet: Protein concentrations were determined using a BCA assay kit and equal amounts of proteins were resolved by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a 0.45 μmpolyvinylidene difluoride (PVDF) membrane (Millipore, CA, USA). .. The membrane was blocked with 5% nonfat milk/Tris-buffered saline (TBS) for 1 h and probed overnight at 4 °C with primary antibodies: anti-GAPDH (TA802519, OriGene, USA), anti-Homer1 (ab184955, Abcam, UK), anti-PSD95 (#3450, CST, USA), anti-Syn38 (ab8049, Abcam, UK), anti-NFATc4 (sc-271597, Santa Cruz, USA), anti-p-NFATc4 (sc-135771, Santa Cruz, USA), anti-GLT1 (#20848, CST, USA), anti-LaminB1 (#13435, CST, USA). .. HRP-linked IgG (H + L) anti-rabbit (#M21002S, 1:5000) and anti-mouse (M21001S, 1:5000) were purchased from Abmart (Shanghai, China).

    Saline:

    Article Title: Reduced astrocytic Homer1a expression mediates mPFC excitatory synaptic dysfunction and neurodevelopmental deficits after maternal immune activation
    Article Snippet: Protein concentrations were determined using a BCA assay kit and equal amounts of proteins were resolved by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a 0.45 μmpolyvinylidene difluoride (PVDF) membrane (Millipore, CA, USA). .. The membrane was blocked with 5% nonfat milk/Tris-buffered saline (TBS) for 1 h and probed overnight at 4 °C with primary antibodies: anti-GAPDH (TA802519, OriGene, USA), anti-Homer1 (ab184955, Abcam, UK), anti-PSD95 (#3450, CST, USA), anti-Syn38 (ab8049, Abcam, UK), anti-NFATc4 (sc-271597, Santa Cruz, USA), anti-p-NFATc4 (sc-135771, Santa Cruz, USA), anti-GLT1 (#20848, CST, USA), anti-LaminB1 (#13435, CST, USA). .. HRP-linked IgG (H + L) anti-rabbit (#M21002S, 1:5000) and anti-mouse (M21001S, 1:5000) were purchased from Abmart (Shanghai, China).

    Transcranial Magnetic Stimulation:

    Article Title: Reduced astrocytic Homer1a expression mediates mPFC excitatory synaptic dysfunction and neurodevelopmental deficits after maternal immune activation
    Article Snippet: Protein concentrations were determined using a BCA assay kit and equal amounts of proteins were resolved by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a 0.45 μmpolyvinylidene difluoride (PVDF) membrane (Millipore, CA, USA). .. The membrane was blocked with 5% nonfat milk/Tris-buffered saline (TBS) for 1 h and probed overnight at 4 °C with primary antibodies: anti-GAPDH (TA802519, OriGene, USA), anti-Homer1 (ab184955, Abcam, UK), anti-PSD95 (#3450, CST, USA), anti-Syn38 (ab8049, Abcam, UK), anti-NFATc4 (sc-271597, Santa Cruz, USA), anti-p-NFATc4 (sc-135771, Santa Cruz, USA), anti-GLT1 (#20848, CST, USA), anti-LaminB1 (#13435, CST, USA). .. HRP-linked IgG (H + L) anti-rabbit (#M21002S, 1:5000) and anti-mouse (M21001S, 1:5000) were purchased from Abmart (Shanghai, China).

    Western Blot:

    Article Title: A small-molecule stabilizer of the calpastatin–calpain-2 complex restores mitochondrial function and mitigates neurodegeneration
    Article Snippet: CHIR99021 (S2924) was from Selleckchem (Houston, TX, USA). .. The antibodies used for the Western blot are listed as follows: Anti–DARPP-32 (ab40801, Abcam, Cambridge, UK, 1:3000), anti-BDNF (ab108319, Abcam, 1:1000), anti-ATPB (ATP synthase, beta subunit) (17247-1-AP, Proteintech, 1:3000), anti-EEA1 (28347-1-AP, Proteintech, 1:3000), anti-tau antibody (10274-1-AP, Proteintech, 1:3000), anti–phospho-tau (Ser 202 and Thr 205 ) (Thermo Fisher Scientific, clone AT8, 1:1000), anti-GSK3 (05-412, MilliporeSigma, 1:1000), anti-Htt protein (MAB5374, clone EM48, MilliporeSigma, 1:1000), anti–β-actin (A1978, MilliporeSigma, 1:10000), anti-DLP1 (611113, BD Bioscience, Franklin Lakes, NJ, USA, 1:2000), anti-PSD95 (2507, Cell Signaling Technology, Danvers, MA, USA, 1:5000), anti–Drp1-pS616 (3455S, Cell Signaling Technology, 1:1000), anti-p35/25 (2680, Cell Signaling Technology, 1:1000), anti-CAST (4146, Cell Signaling Technology, 1:2000), anti–calpain-2 (2539, Cell Signaling Technology, 1:1000), anti–calpain-1 (2556, Cell Signaling Technology, 1:1000), anti–α-spectrin-II (sc-48382, Santa Cruz Biotechnology, Dallas, TX, USA, 1:500), and anti-enolase (sc-15343, Santa Cruz Biotechnology, 1:2000), and horseradish peroxidase (HRP)–conjugated anti-rabbit or anti-mouse IgG (31430/31460, Thermo Fisher Scientific, 1:5000). ..



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    Modulation of synaptic plasticity protein expression by L-lactate and GPR81 agonist in primary hippocampal cells. (a) Exemplary immunoblotting analysis. (b-d) Bar graphs depict the quantified protein levels (normalized to CON) for BDNF, SYN, and <t>PSD95,</t> derived from three independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01 versus the CON group.
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    Image Search Results


    Modulation of synaptic plasticity protein expression by L-lactate and GPR81 agonist in primary hippocampal cells. (a) Exemplary immunoblotting analysis. (b-d) Bar graphs depict the quantified protein levels (normalized to CON) for BDNF, SYN, and PSD95, derived from three independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01 versus the CON group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Lactate promotes mitochondrial biogenesis and fusion via GPR81 to enhance synaptic plasticity-related protein expression in hippocampal cells

    doi: 10.3389/fcell.2026.1929409

    Figure Lengend Snippet: Modulation of synaptic plasticity protein expression by L-lactate and GPR81 agonist in primary hippocampal cells. (a) Exemplary immunoblotting analysis. (b-d) Bar graphs depict the quantified protein levels (normalized to CON) for BDNF, SYN, and PSD95, derived from three independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01 versus the CON group.

    Article Snippet: Following transfer, membranes were blocked and incubated overnight at 4 °C with the indicated primary antibodies: GPR81 (1:400, Sigma, SAB1300790), p-ERK1/2 (1:1000, CST, 4370), ERK1/2 (1:1000, CST, 4695), BDNF (1:1000, Proteintech, 28205-1-AP), SYN (1:1000, CST, 4329), PSD95 (1:1000, CST, 3450), PGC-1α (1:1000, Novus, NBP1-04676SS), NRF1 (1:1000, CST, 46743), NRF2 (1:1000, CST, 12721), TFAM (1:2000, Abcam, ab131607), OPA1 (1:1000, CST, 8047), MFN1 (1:2000, Proteintech, 13798-1-AP), MFN2 (1:1000, CST, 9482), and β-Tubulin (1:2000, Proteintech, 10068-1-AP).

    Techniques: Expressing, Western Blot, Analysis, Derivative Assay

    Effects of mitochondrial dysfunction on expression of genes and proteins related to synaptic plasticity in Neuro-2A cells. (a-e) Quantitative analysis (qRT-PCR) of Arc , Fos , Egr1 , bdnf , and Syp , derived from three independent replicates. (f) Exemplary Western blot image. (g-i) Bar graphs depict the quantified protein levels for BDNF, SYN, and PSD95, derived from six independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the LA group. # p < 0.05, ## p < 0.01, ### p < 0.001 versus the 3OBA group. n. s., no significant difference.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Lactate promotes mitochondrial biogenesis and fusion via GPR81 to enhance synaptic plasticity-related protein expression in hippocampal cells

    doi: 10.3389/fcell.2026.1929409

    Figure Lengend Snippet: Effects of mitochondrial dysfunction on expression of genes and proteins related to synaptic plasticity in Neuro-2A cells. (a-e) Quantitative analysis (qRT-PCR) of Arc , Fos , Egr1 , bdnf , and Syp , derived from three independent replicates. (f) Exemplary Western blot image. (g-i) Bar graphs depict the quantified protein levels for BDNF, SYN, and PSD95, derived from six independent replicates. Data are expressed as mean ± SD. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001 versus the LA group. # p < 0.05, ## p < 0.01, ### p < 0.001 versus the 3OBA group. n. s., no significant difference.

    Article Snippet: Following transfer, membranes were blocked and incubated overnight at 4 °C with the indicated primary antibodies: GPR81 (1:400, Sigma, SAB1300790), p-ERK1/2 (1:1000, CST, 4370), ERK1/2 (1:1000, CST, 4695), BDNF (1:1000, Proteintech, 28205-1-AP), SYN (1:1000, CST, 4329), PSD95 (1:1000, CST, 3450), PGC-1α (1:1000, Novus, NBP1-04676SS), NRF1 (1:1000, CST, 46743), NRF2 (1:1000, CST, 12721), TFAM (1:2000, Abcam, ab131607), OPA1 (1:1000, CST, 8047), MFN1 (1:2000, Proteintech, 13798-1-AP), MFN2 (1:1000, CST, 9482), and β-Tubulin (1:2000, Proteintech, 10068-1-AP).

    Techniques: Expressing, Analysis, Quantitative RT-PCR, Derivative Assay, Western Blot